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linzhao cheng  (Addgene inc)


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    Structured Review

    Addgene inc linzhao cheng
    Linzhao Cheng, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 62 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/linzhao+cheng/EF%2ESTAT3C%2EUbc%2EGFP+(Plasmid+%2324983)/pmc12371199-150-11-13
    Average 93 stars, based on 62 article reviews
    linzhao cheng - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Reactive astrocytes transduce inflammation in a blood-brain barrier model through a TNF-STAT3 signaling axis and secretion of alpha 1-antichymotrypsin.
    Article Snippet: Viral particles were collected at 72 h by filtering spent medium through a 0.45μm cellulose acetate filter (Millipore Sigma), then concentrating the supernatant with AAVpro® concentrator (TaKaRa). .. Plasmid EF.STAT3DN.UBC.GFP, which contains the cDNA encoding for the dominant-negative form of human STAT3 (STAT3DN), was a gift from Linzhao Cheng (Addgene plasmid #24984). .. Plasmid pMK1334 for lentiviral delivery of sgRNA was a gift from Martin Kampmann (Addgene plasmid #127965).

    Article Title: Identification of c‐Met on Tumor Cells as a Novel Receptor for B7‐H3 Entails Implications for Cancer Cell Stemness and Targeted Therapy
    Article Snippet: All the lentiviral vectors used in this study were synthesized by Genechem (Shanghai, China). .. Moreover, plasmid EF.STAT3C.Ubc.GFP constitutively expressed active STAT3 was a gift from Linzhao Cheng (Addgene plasmid # 24983, Cambridge, MA, USA). .. Protein was extracted from the cells using 1% SDS lysis buffer (62.5 mM Tris‐HCl, 2% w/v SDS, 10% glycerol, 50 mM DTT, 0.01% w/v bromophenol blue) supplemented with protease inhibitor cocktail (100×; Beyotime, Shanghai, People's Republic of China), resolved by SDS‐polyacrylamide gels and then transferred to PVDF membranes (0.45 μm, Millipore, Billerica, MA, USA).

    Article Title: The dynamics of chemoattractant receptors redistribution in the electrotaxis of 3T3 fibroblasts.
    Article Snippet: .. To obtain 3T3 fibroblasts stably expressing GFP, cells were transduced with a pEGIP lentiviral vector which was a gift from Linzhao Cheng (Addgene plasmid # 26777) [32] according to the protocol described before [29], followed with antibiotic selection using 10 μg/ml puromycin (Sigma-Aldrich, St. Louis, MO, USA). ..

    Article Title: Cancer-associated fibroblasts promote cancer stemness by inducing expression of the chromatin-modifying protein CBX4 in squamous cell carcinoma.
    Article Snippet: STATTIC (2798) was purchased from Tocris and UNC3866 (19237) was purchased from Cayman Chemical. .. STAT3C.Ubc.GFP was a gift from Linzhao Cheng (Addgene plasmid # 24983; http://n2t.net/ addgene:24983; RRID:Addgene_24983 (20)). .. ∆Np63α-FLAG was a gift from David Sidransky (Addgene plasmid # 26979; http://n2t.net/addgene:26979; RRID: Addgene_26979 (21)).

    Article Title: BRD4 Regulates Transcription Factor ΔNp63α to Drive a Cancer Stem Cell Phenotype in Squamous Cell Carcinomas
    Article Snippet: .. STAT3C.Ubc.GFP was a gift from Linzhao Cheng (Addgene plasmid # 24983; http://n2t.net/addgene:24983; RRID:Addgene_24983; ref. 14). .. DeltaNp63alpha-FLAG was a gift from David Sidransky (Addgene plasmid # 26979; http://n2t.net/addgene:26979; RRID: Addgene_26979; ref. 15). pcDNA3-cmyc was a gift from Wafik ElDeiry (Addgene plasmid # 16011; http://n2t.net/addgene:16011; RRID:Addgene_16011; ref. 16).

    Article Title: Niraparib-induced STAT3 inhibition increases its antitumor effects.
    Article Snippet: .. The EF.STAT3C.Ubc.GFP plasmid was a gift from Linzhao Cheng (RRID: Addgene_24983; http://n2t.net/addgene:24983). .. The FUGW plasmid was a gift from David Baltimore (RRID: Addgene_14883; http://n2t.net/addgene:14883).

    Article Title: Direct inhibition of the TLR4/MyD88 pathway by geniposide suppresses HIF-1α-independent VEGF expression and angiogenesis in hepatocellular carcinoma.
    Article Snippet: In detail, the contributors of these specific plasmids were listed as follows: the pGL4.10-VEGFprom was a gift from David Mu (Addgene Plasmid # 66128; http://n2t.net/addgene:66128; RRID: Addgene_66128; Wood et al., 2016); the pN3-Sp1FL was a gift from Guntram Suske (Addgene Plasmid # 24543; http://n2t.net/ addgene:24543; RRID:Addgene_24543); and the EF.STAT3C.Ubc. .. GFP was a gift from Linzhao Cheng (Addgene Plasmid # 24983; http://n2t.net/addgene: 24983; RRID:Addgene_24983; Hillion et al., 2008). .. Transfection was performed according to the manufacturer's instruction regarding the application of FuGene reagent (Promega, USA).

    Article Title: An in vitro platform for quantifying cell cycle phase lengths in primary human intestinal epithelial cells.
    Article Snippet: Plasmids were subsequently harvested from bacterial stocks using the QIAGEN HiSpeed Maxi kit [Qiagen 12662]. .. The PIP-FUCCI plasmid11 was a gift from Jean Cook and Jeremy Purvis. pPIGA-PHD was a gift from Linzhao Cheng [Addgene plasmid #26778; http:// n2t. net/ addge ne: 26778; RRID:Addgene_26778]37. sg resistant gammatubulin was a gift from Maria Alvarado-Kristensson [Addgene plasmid #104433; http:// n2t. net/ addge ne: 104433; RRID:Addgene_104433], and pmScarlet_H2A_C1 was a gift from Dorus Gadella [Addgene plasmid #85051; http:// n2t. net/ addge ne: 85051; RRID:Addgene_85051]. .. Plasmids were introduced into human cells using our optimized protocol20 employing the Neon Transfection System 100 μL Kit [ThermoFisher MPK10096].

    Dominant Negative Mutation:

    Article Title: Reactive astrocytes transduce inflammation in a blood-brain barrier model through a TNF-STAT3 signaling axis and secretion of alpha 1-antichymotrypsin.
    Article Snippet: Viral particles were collected at 72 h by filtering spent medium through a 0.45μm cellulose acetate filter (Millipore Sigma), then concentrating the supernatant with AAVpro® concentrator (TaKaRa). .. Plasmid EF.STAT3DN.UBC.GFP, which contains the cDNA encoding for the dominant-negative form of human STAT3 (STAT3DN), was a gift from Linzhao Cheng (Addgene plasmid #24984). .. Plasmid pMK1334 for lentiviral delivery of sgRNA was a gift from Martin Kampmann (Addgene plasmid #127965).

    Stable Transfection:

    Article Title: The dynamics of chemoattractant receptors redistribution in the electrotaxis of 3T3 fibroblasts.
    Article Snippet: .. To obtain 3T3 fibroblasts stably expressing GFP, cells were transduced with a pEGIP lentiviral vector which was a gift from Linzhao Cheng (Addgene plasmid # 26777) [32] according to the protocol described before [29], followed with antibiotic selection using 10 μg/ml puromycin (Sigma-Aldrich, St. Louis, MO, USA). ..

    Expressing:

    Article Title: The dynamics of chemoattractant receptors redistribution in the electrotaxis of 3T3 fibroblasts.
    Article Snippet: .. To obtain 3T3 fibroblasts stably expressing GFP, cells were transduced with a pEGIP lentiviral vector which was a gift from Linzhao Cheng (Addgene plasmid # 26777) [32] according to the protocol described before [29], followed with antibiotic selection using 10 μg/ml puromycin (Sigma-Aldrich, St. Louis, MO, USA). ..

    Transduction:

    Article Title: The dynamics of chemoattractant receptors redistribution in the electrotaxis of 3T3 fibroblasts.
    Article Snippet: .. To obtain 3T3 fibroblasts stably expressing GFP, cells were transduced with a pEGIP lentiviral vector which was a gift from Linzhao Cheng (Addgene plasmid # 26777) [32] according to the protocol described before [29], followed with antibiotic selection using 10 μg/ml puromycin (Sigma-Aldrich, St. Louis, MO, USA). ..

    Selection:

    Article Title: The dynamics of chemoattractant receptors redistribution in the electrotaxis of 3T3 fibroblasts.
    Article Snippet: .. To obtain 3T3 fibroblasts stably expressing GFP, cells were transduced with a pEGIP lentiviral vector which was a gift from Linzhao Cheng (Addgene plasmid # 26777) [32] according to the protocol described before [29], followed with antibiotic selection using 10 μg/ml puromycin (Sigma-Aldrich, St. Louis, MO, USA). ..



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